overexpression system (OriGene)
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Structured Review
OriGene
overexpression system
Overexpression System, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cept1/CEPT1+(NM_001007794)+Human+Over-expression+Lysate/pmc12784299-80-26-31
Average 94 stars, based on 2 article reviews
Overexpression System, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cept1/CEPT1+(NM_001007794)+Human+Over-expression+Lysate/pmc12784299-80-26-31
Average 94 stars, based on 2 article reviews
overexpression system - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Endothelial CEPT1 Promotes Angiogenesis Through PPARα and VEGF-A Signaling"
Article Title: Endothelial CEPT1 Promotes Angiogenesis Through PPARα and VEGF-A Signaling
Journal: Arteriosclerosis, thrombosis, and vascular biology
doi: 10.1161/ATVBAHA.125.323302
Figure Legend Snippet: A ) Experimental design of aortic cell isolation for scRNA-seq analysis. B ) t-distributed stochastic neighbor embedding of EC and stromal cells separated by genotypes. C ) Pie-chart representing the relative abundance of each cluster. D ) Violin plot of Cept1 gene expression in EC sub-clusters (EC 1–3). E ) Pathway analysis of Differential Expressed Genes (DEG) between Cept1 overexpression and control in EC1 sub-cluster (FDR adjusted p value of <0.05, log (FC) > 0.1 and pct.1 >0.6). F-H ) Module scores based on significantly upregulated pathways.
Techniques Used: Over Expression, Cell Isolation, Gene Expression, Control
Figure Legend Snippet: A ) Graphical representation of the experiment design. B ) Representative Doppler perfusion of HLI of Cept1 fl/fl Cre − and Cept1 fl/fl Cre + mice (n=4–5 mice) treated with STZ. C-D ) Quantitative graphical representation of Doppler perfusion of the hindpaw and gastrocnemius muscle. E-F ) Limb and ischemic damage severity were evaluated on days 3, 7, 14, and 21. G ) Representative images showing HE, Isolectin B4 (IB4), and CD31 staining in the gastrocnemius muscle at 21 days after artery ligation (n=4–5 mice per group). Scale bar =50 μm. H-I ) Quantification of relative microvessel density, relative muscle fiber area CD31 staining (n=4–5 mice per group). Data are presented as mean ± SEM. Statistical analysis in panel C-F was performed using multiple t -test with FDR by the 2-stage step-up method of Benjamini, Krieger, and Yekutieli multiplicity adjustment. Statistical analysis in panel H-J was performed using an unpaired Student’s test.
Techniques Used: Over Expression, Staining, Ligation
Related Articles
Concentration Assay:Article Title: Identification of a Physiologically Relevant Endogenous Ligand for PPAR? in Liver Article Snippet: .. For siRNA experiments, 50–60% confluent cells were treated with siRNAs or their scrambled controls (all diluted in PBS) for 72 h. Following the 72 h siRNA treatment, another set of cells received 16:0/18:1-GPC (using a concentration based on dose-response experiments) for an additional 24 h. For overexpression of CEPT1, cells were transfected with a vector containing Over Expression:Article Title: Identification of a Physiologically Relevant Endogenous Ligand for PPAR? in Liver Article Snippet: .. For siRNA experiments, 50–60% confluent cells were treated with siRNAs or their scrambled controls (all diluted in PBS) for 72 h. Following the 72 h siRNA treatment, another set of cells received 16:0/18:1-GPC (using a concentration based on dose-response experiments) for an additional 24 h. For overexpression of CEPT1, cells were transfected with a vector containing Transfection:Article Title: Identification of a Physiologically Relevant Endogenous Ligand for PPAR? in Liver Article Snippet: .. For siRNA experiments, 50–60% confluent cells were treated with siRNAs or their scrambled controls (all diluted in PBS) for 72 h. Following the 72 h siRNA treatment, another set of cells received 16:0/18:1-GPC (using a concentration based on dose-response experiments) for an additional 24 h. For overexpression of CEPT1, cells were transfected with a vector containing Clone Assay:Article Title: Identification of a Physiologically Relevant Endogenous Ligand for PPAR? in Liver Article Snippet: .. For siRNA experiments, 50–60% confluent cells were treated with siRNAs or their scrambled controls (all diluted in PBS) for 72 h. Following the 72 h siRNA treatment, another set of cells received 16:0/18:1-GPC (using a concentration based on dose-response experiments) for an additional 24 h. For overexpression of CEPT1, cells were transfected with a vector containing Plasmid Preparation:Article Title: Identification of a Physiologically Relevant Endogenous Ligand for PPAR? in Liver Article Snippet: .. For siRNA experiments, 50–60% confluent cells were treated with siRNAs or their scrambled controls (all diluted in PBS) for 72 h. Following the 72 h siRNA treatment, another set of cells received 16:0/18:1-GPC (using a concentration based on dose-response experiments) for an additional 24 h. For overexpression of CEPT1, cells were transfected with a vector containing Stable Transfection:Article Title: Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets Article Snippet: The siRNAs were transfected using Lipofectamine RNAiMAX (Thermo Fisher). .. For Generated:Article Title: Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets Article Snippet: The siRNAs were transfected using Lipofectamine RNAiMAX (Thermo Fisher). .. For Retroviral:Article Title: Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets Article Snippet: The siRNAs were transfected using Lipofectamine RNAiMAX (Thermo Fisher). .. For |